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Ateh1/pan1

WebMar 25, 2024 · RCSB PDB - 6YET: Second EH domain of AtEH1/Pan1 Structure Summary NMR Ensemble 3D View : Structure 1D-3D View Validation Report Ligand Interaction Macromolecule Content Total Structure Weight: 12.38 kDa Atom Count: 858 Modelled Residue Count: 104 Deposited Residue Count: 106 Unique protein chains: 1 Display … WebApr 23, 2024 · Collectively, our structural and functional studies reveal distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connect the internalization of SCAMP5 to the TPLATE complex. AtEH/Pan1 proteins contain two N-terminal Eps15 homology (EH) domains and are subunits of the endocytic TPLATE …

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WebAug 13, 2024 · In Arabidopsis, AtEH/Pan1 proteins play a dual role, where on one hand, they drive actin-mediated autophagy, and on the other hand, they bind auxiliary endocytic adaptors as well as the plasma membrane (Wang et al., 2024; Yperman et al., 2024). WebMay 24, 2024 · The AtEH/Pan1 proteins are the plant homologs of yeast Pan1p, which is known for its role as an activator of ARP2/3-dependent actin dynamics during endocytosis 9 – 11. Pan1p was also recently shown to be part of a phosphorylation-dependent mechanism connecting endocytic vesicles, endosomal compartments, and actin dynamics in budding … gasthof hubertusstube st. johann https://zenithbnk-ng.com

Distinct EH domains of the endocytic TPLATE complex confer …

WebAtEH1/Pan1 and AtEH2/Pan1 are essential for plant life. In AtEH1/Pan1 heterozygous mutants, shrivelled and normal looking pollen grains are observed in a ratio of 1:1 (Fig. 2a), similar to... WebUtilizing advanced time-lapse imaging and kymograph analysis, the authors show that the TPLATE, AtEH1/Pan1, and AtEH2/Pan1 TPC subunits are recruited to the PM simultaneously, at both 25°C and 12°C ( Fig. 1 ). WebIn particular, two TPC members, AtEH1/Pan1 and AtEH2/Pan1 play an important role in this pathway (Figure 8) and several components of the endocytic machinery are associated with this pathway, either as machinery or as cargo. Ongoing research focusses on unravelling in detail how this pathway is initiated, as well as how it is connected to the ... david schink obituary

High Temporal Resolution Reveals Simultaneous Plasma …

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Ateh1/pan1

Plant AtEH/Pan1 proteins drive autophagosome …

WebSep 1, 2024 · AtEH1/Pan1 contains a typical VCA motif, which is responsible for ARP2/3 activation; it is able to bind to actin filaments and interact with APR2/3 subunits, … WebYeast transformed with pGBKT7-S7-2/pGADT7 or pGBKT7/pGADT7-SKP1 Maize used as negative controls were unable to grow. Techniques Used: Positive Control, Sequencing, Construct, Transformation Assay control plasmids pgbkt7 p53 ( TaKaRa ) TaKaRa is a verified supplier TaKaRa manufactures this product About News Press Release Team …

Ateh1/pan1

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WebThe Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are … WebApr 25, 2024 · NAP1 (SCAR/WAVE)-regulated autophagy responds to multiple autophagy stresses, such as high salt and starvation. Interestingly, NAP1 also responds to constant mechanical pressure, which can induce massive autophagosome formation when cells are put under pressure [ 7. , 38. , 39. ].

WebDec 14, 2024 · The two EH domains of atEH1, EH1.1 and EH1.2 as defined by ref. 21, were amplified from synthetic AtEH1/Pan1 (codon optimized for bacterial expression, IDT) (SI … WebMay 24, 2024 · Structural characterization of both EH domains of AtEH1/Pan1 reveals a common fold. AtEH/Pan1 proteins are highly unstructured but three domains can be …

WebMar 17, 2024 · AtEH1/Pan1 and AtEH2/Pan1 are plant-specific subunits of TPC that are connected to the other six subunits via a physical interaction with the TML subunit … WebNov 13, 2024 · The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. …

WebThe TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and …

WebJan 28, 2024 · Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion... david schipper men\u0027s healthWebMar 17, 2024 · Our results reveal that AtEH/Pan1 proteins act as scaffolds to direct endocytic machinery to specific plasma membrane regions to initiate internalisation. These findings provide new insight into the interplay between membranes and protein condensates. Competing Interest Statement The authors have declared no competing … david schirmer deathWebApr 9, 2024 · The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endo-cytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homo-logues of the yeast ARP2/3 ... gasthof indianaWebMar 18, 2024 · Clathrin-mediated endocytosis (CME) is an essential cellular internalisation pathway involving the dynamic assembly of clathrin and accessory proteins to form … gasthof inchenhofendavid schipper men\\u0027s healthWebMoreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either … gasthof in sterzing südtirolWebApr 1, 2024 · Title: Crystal structure of the N-terminal EF-hand domain of Arabidopsis thaliana AtEH1/Pan1 DOI: 10.2210/pdb6yig/pdb Primary publication DOI: 10.1038/s41467-021-23314-6 gasthof innichen