Myc-tagged full-length human Rootletin was a gift from Erich Nigg (Addgene plasmid # 41167)24. In a previous study, R1, R123, and R234-deletion constructs were generated from mouse Rootletin and GFP-tagged35; these constructs were thoroughly humanized, and the GFP tag was replaced with Myc or … See more A schematic of our custom-made CNOM16 is shown in Fig. S2a. Owing to recent advances in supercontinuum (SC) generation technology, our CNOM system has extended to ultra-broadband spectroscopic live cell … See more Because the excitation wavelength of the original custom-made penta-modal CNOM was ≥1064 nm, we could not excite typical fluorophores such as Alexa Fluor 488. In order to achieve simultaneous measurement of SHG … See more Antibodies were obtained from the indicated suppliers: goat anti-Rootletin for immunostaining (sc-67824) and mouse anti-Rootletin for immunoblotting (sc-374056; Santa Cruz … See more COS7 and U2OS cells (ATCC, Manassas, VA) were cultured in DMEM containing 10% fetal bovine serum. They were authenticated by species-specific PCR49 or by short tandem repeat (STR) profiling techniques, … See more
Entry - *615776 - CILIARY ROOTLET COILED-COIL PROTEIN; …
Web12 Jun 2024 · Rootletin is the main component of the ciliary rootlet and functions as a centriole linker connecting the two mother centrioles. Despite the functional importance of rootletin, the molecular architecture of the rootletin filament and its assembly mechanism are poorly understood. Here, we identify th … Web23 Aug 2024 · severe redness, swelling, blistering, peeling, or crusting; Your skin may be more sensitive to weather extremes such as cold and wind while using this medicine. … dari informatics services pvt. ltd
Focus on Molecules: Rootletin Request PDF - ResearchGate
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